Effects of the wavelength of light, photoperiod, and salinity in the production parameters of Isochrysis galbana
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Abstract
Production of Isochrysis galbana is one of the fundamental steps in oyster farms. The nutritional quality of this microalgae is very important, from this will depend the optimal development of the larvae, since in Costa Rica oyster culturing is growing, optimizing the microalgal culture has become a necessity not only in terms of producing more biomass but also to reduce the cost associated to it. For the bioassays, bottles with a capacity of 5.5 L were used, a total of 6 tests were carried out, three with a wavelength of white light (500 nm) and three with a wavelength of blue light (455 nm), at each wavelength three photoperiods were tested 16h:8h, 12h:12h and 24h (Light/Dark) and 4 salinities (32ppm, 30ppm, 28ppm and 25ppm). After 12 days of culture, it was observed that the highest number of cells/ml were obtained in the blue light treatment, with constant irradiation 24h and salinity of 28ppm. The photoperiod of 12h:12h (L/0) did not have a good effect on growth in any of the wavelengths of light used. Regarding biomass production, it was determined that the blue light treatment, photoperiod 16h:8h (L/O) and salinity of 30ppm was the one with the highest weight of dry biomass. The bioassays made it possible to determine that the combination of blue light, with constant irradiation 24h and salinity of 28ppm, showed the highest cell density.
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