Case Report: detection and characterization of the tomato yellow leaf curl virus (TYLCV) in tomato plants from Sarchí, Costa Rica
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Abstract
The present study focused on investigating the presence of tomato yellow leaf curl virus (TYLCV)
in tomato plants in Sarchí, Costa Rica. Samples from plants with symptoms of the disease, as
well as a sample without symptoms, were collected, and molecular and microscopic analysis
were performed to detect and characterize the virus. The results confirmed the presence of
TYLCV in the analyzed samples by PCR amplification and by comparison with other sequences
using a BLAST and a phylogenetic tree. In addition, differences in the severity of symptoms were
observed between plants exposed to sun or in the shade. Cytopathological symptoms were
identified by transmission electron microscopy in the chloroplasts of infected plants, such as
deformation, presence of starch granules, and changes in the thylakoid structure. These findings
confirm the presence of TYLCV in the tomato crops analyzed in this study. These results may be
useful for farmers and scientists seeking to develop management and control strategies for this
disease in tomato crops in Costa Rica.
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