Raman spectroscopy analysis of DNA using the SERS method
Article Sidebar
Main Article Content
Abstract
The technological advances of the last decades have contributed to the development of new methodologies for the characterization of biological samples. Raman Spectroscopy has been used for the characterization of pure inorganic and organic compounds, but recently, it is being used to study molecular structures of biological importance. Fluorescence is usually present in Raman spectroscopy, shielding Raman signals, but using the SERS (Surface Enhanced Raman Spectroscopy) technique, it is possible to locally increase the Raman signal and to reduce the importance of the fluorescence effects on the samples. The SERS effect could be achieved by adding metallic nanoparticles, of a specific size distribution, to the sample. Usually gold and silver nanoparticles are used for SERS. In this research, DNA of human and Rickettsia bacteria were characterized by the SERS technique, with both silver and gold nanoparticles.
Article Details
Los autores conservan los derechos de autor y ceden a la revista el derecho de la primera publicación y pueda editarlo, reproducirlo, distribuirlo, exhibirlo y comunicarlo en el país y en el extranjero mediante medios impresos y electrónicos. Asimismo, asumen el compromiso sobre cualquier litigio o reclamación relacionada con derechos de propiedad intelectual, exonerando de responsabilidad a la Editorial Tecnológica de Costa Rica. Además, se establece que los autores pueden realizar otros acuerdos contractuales independientes y adicionales para la distribución no exclusiva de la versión del artículo publicado en esta revista (p. ej., incluirlo en un repositorio institucional o publicarlo en un libro) siempre que indiquen claramente que el trabajo se publicó por primera vez en esta revista.
References
[2] A. A. Bunaciu, H. Y. Aboul-Enein and V. D. Hoang, “Raman Spectroscopy for Protein Analysis”, Applied Spectroscopy Reviews, vol. 50, no. 5, pp. 377-386, 2015.
[3] M. Vendrell, K. Maiti, K. Dhaliwal and Y. Chang, “Surface-enhanced Raman scattering in cancer detection and imaging”, Trends in Biotechnology, vol. 31, no. 4, pp. 249-257, 2013.
[4] F. Gao, J. Lei and H. Ju, “Label-Free Surface-Enhanced Raman Spectroscopy for Sensitive DNA Detection by DNA-Mediated Silver Nanoparticle Growth”, Analytical Chemistry, vol. 85, no. 24, pp. 11788-11793, 2013.
[5] P. Chandra. “10.11.2 Optical Fibre”, Nanobiosensors for Personalized and Onsite Biomedical Diagnosis. Institution of Engineering and Technology, 2016.
[6] R. Aroca, Amplificación plasmónica de espectros Raman y de fluorescencia: SERS y SEF sobre nanoestructuras metálicas. Editorial CSIC Consejo Superior de Investigaciones Científicas, 2014.
[7] P. Lee and D. Meisel, “Adsorption and surface-enhanced Raman of dyes on silver and gold sols”, The Journal of Physical Chemistry, vol. 86, no. 17, pp. 3391-3395, 1982.
[8] A. Corzo, “Síntesis de Nanopartículas de Oro Obtenidas por Reducción de H[AuCl4]”, Rev Soc Quim Perú, pp. 79-79, 2012.
[9] P. L. Stiles, J. A. Dieringer, N. C. Shah y R. P. Van Duyne, “Surface-Enhanced Raman Spectroscopy,” Annual Review of Analytical Chemistry, vol. 1, p. 601–626, 2008.
[10] L. Qiu, P. Liu, L. Zhao, M. Wen, H. Yang, S. Fan and L. Zhou, “Analysis of plant genomic DNAs and the genetic relationship among plants by using surface-enhanced Raman spectroscopy”, Vibrational Spectroscopy, vol. 72, pp. 134-141, 2014.
[11] J. Sundaram, B. Park, A. Hinton, K. Lawrence and Y. Kwon, “Detection and differentiation of Salmonella serotypes using surface enhanced Raman scattering (SERS) technique”, Journal of Food Measurement and Characterization, vol. 7, no. 1, pp. 1-12, 2013.
[12] X. Li, T. Yang and J. Lin, “Spectral analysis of human saliva for detection of lung cancer using surface-enhanced Raman spectroscopy”, Journal Of Biomedical Optics, vol. 17, no. 3, p. 037003, 2012.
[13] R. Draga, M. Grimbergen, P. Viiverberg, C. Swol, T. Jonges, J. Kummer and J. Ruud Bosch, “In Vivo Bladder Cancer Diagnosis by High-Volume Raman Spectroscopy”, Analytical Chemistry, vol. 82, no. 14, pp. 5993-5999, 2010.
[14] C. Muntean, N. Leopold, C. Tripon, A. Coste and A. Halmagyi, “Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation”, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol. 144, pp. 107-114, 2015.
[15] K. Virkler and I. Lednev, “Raman spectroscopic signature of blood and its potential application to forensic body fluid identification”, Analytical And Bioanalytical Chemistry, vol. 396, no. 1, pp. 525-534, 2009.