Efficiency in reducing lignocellulose, caffeine and tannins in coffee husks (Coffea arabica) ensiled and depleted by Pleurotus djamor

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José Alfonso López-García
Jacqueline Toledo-Roblero
Victor Jesus Albores Flores
María Guadalupe de Gyves-Córdova
José Arturo Oleta-Barrios
Julieta Grajales-Conesa

Abstract

Introduction. Coffee husks have limitations for their use in animal feed due to the content of tannins, caffeine and lignin, which requires bioconversion with microorganisms, such as silage and depletion of the substrate with fungi that allow the bioconversion of lignocellulosic waste. Objective. To evaluate the effectiveness of coffee husk silage in reducing the caffeine and tannin content and the decrease in lignocellulolytic compounds due to substrate depletion with Pleurotus djamor. Materials and methods. In this study, two treatments of molasses were carried out for silage, 5% and 10% molasses, left for 160 days. Samples were taken in triplicate for chemical analysis on day 0 and at the end of ensiling, reducing sugars were determined. , protein, ash and fiber according to standardized procedures, cellulose by Kurschner and Hoffer, lignin by TAPPI 222 om-88, tannins by the FolinCiocalteu method, based on Makkar et al. A completely randomized design with a factorial arrangement was used, ANOVA and Tukey’s test were performed for the comparison of means, and calculations were also performed to determine the effectiveness of the treatments. Results. The silage process significantly reduced caffeine and tannins for both treatments, lignin was reduced by 55.87 and 43.97% respectively in each treatment. Conclusions.  Bioconversion with P. djamor managed to reduce this compound, therefore, coffee husks become feasible as animal supplementation.

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How to Cite
López-García , J. A., Toledo-Roblero , J., Albores Flores, V. J., de Gyves-Córdova, M. G., Oleta-Barrios, J. A., & Grajales-Conesa, J. (2025). Efficiency in reducing lignocellulose, caffeine and tannins in coffee husks (Coffea arabica) ensiled and depleted by Pleurotus djamor. Tecnología En Marcha Journal, 39(1), Pág. 3–13. https://doi.org/10.18845/tm.v39i1.7855
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Artículo científico

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