Bone tissue processing and sterilization for therapeutic use: preclinical experiments from a technological university
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Abstract
Gamma irradiation is among the most widely used methods of bone sterilization. The usual
standard dose for achieving sterilization is 25 kGy. Although there are international standards for
the main procedures, the general recommendation is to validate them at the local level, so that
they are feasible with the personnel and technical capacities of the country. In this study, porcine
bone tissue samples were treated by chemical disinfection and by sterilization with gamma
irradiation, reducing the microbial load to non-detectable levels, without significantly affecting
the desirable properties of the tissue matrix, which was verified by protein characterization of
collagen and bone morphogenetic proteins (BMPs) and by scanning electron microscopy. This
research demonstrates the ability of Costa Rica Institute of Technology (ITCR) to contribute to
the training of professionals and to provide access to specialized equipment, which allows the
strengthening of tissue banks in the country, and thereby contributing to the development of new
technological strategies for human health.
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