Development and characterization of biomaterials for biomimetic tissues applications

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Jeimmy González-Masís
Jorge M. Cubero-Sesin
José Roberto Vega-Baudrit
Rodolfo J. González-Paz


To create artificial tissues, a matrix is needed as structural support. It must be biodegradable, and it must cause the minimal inflammation response in the human body. However, it is important to consider the cost, which usually is very high, and the materials used: their origin and properties. In this work, animal waste and plant extracts were used to generate a biomaterial that is part of a multiphase system from type I collagen as a temporary support, taken from rat tails; which can also be used as a nanoparticles distributor. Those nanoparticles were taken from a plant, called Tinospora cordifolia, and also from a waxy resin obtained from bee hives, called propolis, both used as regenerative agents. It was necessary to prepare and characterize the collagen and nanoparticles using Polyacrylamide gel electrophoresis (SDS- Page), Amplitud Modulated Atomic Force Microscopy (AFM) and Thermogravimetric Analysis (TGA). Skin cells behavior with the nanoparticles was evaluated too. Collagen obtained has a correct structural conformation, with a 65nm D-periodic and repeated sequences of triplets Gly-Pro-Pro (GPP) and a denaturalization temperature from 75 to 85ºC. Nanoparticles from natural extracts have spherical shapes, in sizes of 13.8 nm in average from tinospora and 15 nm from propolis. They produce positive effects on the regeneration of the cells in study. The results show that it is possible to produce a biopolymeric prototype that induce cell self-regeneration from natural materials, as a useful solution in the treatment of skin diseases.

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